300x - Darkfield Algae

10 months ago by cybervseas to c/microscopy

This is my first ever attempt at darkfield microscopy. I could only get my 10x objective working with my makeshift darkfield setup, and at that magnification my single-celled algae look like beauti...

My green water culture is challenging to study with the microscope but still a lot of fun.

pebbles 2 points 10 months ago

Looks great! I feel the pain on trying to get higher magnification darkfield to work lol.

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cybervseas 2 points 10 months ago

I think I need to get my objective and condenser aligned better to get this to work at higher magnifications. I wish I understood how to do that. Right now otherwise at 40x objective only the specimen near the top of the fov will actually be visible. And also I get a gradient on the background instead of solid dark field.

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pebbles 2 points 10 months ago

Ah I didn't realize that aligning the condenser could help. I'm going to try that. I tend to get gradients in my background on higher magnifications as well, but since I do more artsy things I tend to use that to my advantage with rhienburg illumination mixed in. It can look quite nice.

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cybervseas 2 points 10 months ago

Look i say this, but remember i barely know anything here. Still not sure even sure how all those parts are connected. But for setting up Kohler illumination it looks to be essential.

Yesterday I properly aligned the light source on my microscope and wow it was a tremendous improvement in available light. Feels at least 50% brighter.

https://evidentscientific.com/...

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